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Generation of RRV gene 5 variants by serial passage in MA104 cells
| MA104 cells (clones) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| High MOI |
Low moi |
||||||||||
| FRhL2 cells | (48) | Pass 1 | (27) | Pass 2 | (28) | Pass 3 | (40) | Pass 5 | (36) | Pass 5 | (31) |
| UGUGACC | 48 | UGUGACC | 23 | UGUGACC | 18 | UGUGACC | 19 | UGUuauCC | 24 | UGUGACC | 29 |
| UGUuuCC | 2 | UGUu-CC | 4 | UGUu-CC | 8 | UGUu-CC | 6 | UGUuuCC | 1 | ||
| UGUu-CC | 2 | UGUuuCC | 3 | UGUuuCC | 7 | UGUuuCC | 4 | UGUugCC | 1 | ||
| UGUuACC | 1 | UGUuauCC | 2 | UGcu-CC | 1 | ||||||
| UGUuauCC | 1 | UGccuCC | 2 | UGU–CC | 1 | ||||||
| UGUuuuuCC | 1 | UGUauuCC | 1 | ||||||||
| UGUGAaCC | 1 | ||||||||||
