Fig. 2.
Identifying primary GR-regulated genes and mapping their GR-binding regions. A549 cells were treated with ethanol or dexamethasone for 90 min, and ChIP-scanning experiments were performed on GILZ (A), THBD (B), SDPR (C), SLC19A2 (D), PPG (E), BHLHB2 (F), GEM (G), and SNK (H). The PCR primers for ChIP scanning that correspond to distinct regions in each promoter are indicated. The fold enrichment values for the experimental regions were determined by normalizing to the internal control hsp70 gene value. Hatched bars indicate the scanning regions, which were enriched >2-fold. Data represent the SEM of the fold enrichment (dexamethasone-treated cells divided by ethanol-treated cells) from at least three experiments.