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. 2016 Oct 3;44(20):9565–9577. doi: 10.1093/nar/gkw890

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — PA-Apt1 2D predicted structure and binding to PA. (Left) Percentage of aptamer bound versus PA63 (upper graph) or PA83 (lower graph) concentration obtained in by filter binding assays. The inset in PA63 plot shows calculated binding parameters. Binding parameters for PA83 could not be calculated because signals were below detection level. Plotted are the mean values of three filter-binding assay replicas. (Right) The predicted secondary structure of the PA-Apt1 calculated with KineFold. Nucleotides in the primer binding regions are in light gray circles.