Table S2.
Infection of tau-expressing mammalian cells with tauopathy patient samples
| Patient sample | Brain region | Mean cell infection ± SD, fluorescence per cell, 103 A.U.† | |||
| Tau(4RD*LM)–YFP(1)‡ | Tau(3RD*VM)–YFP‡ | Tau(3RD*VM,4RD*LM)–YFP‡ | Tau(4RD*LM)–YFP(2)§ | ||
| C9 | Putamen | 2.0 ± 1.4 | 3.4 ± 1.8 | 6.2 ± 1.6 | 1.0 ± 0.4 |
| C10 | Putamen | 1.6 ± 0.3 | 1.8 ± 0.6 | 4.2 ± 1.7 | 2.5 ± 2.1 |
| C16 | Putamen | 1.3 ± 0.5 | 3.3 ± 1.2 | 4.9 ± 2.3 | 1.4 ± 1.2 |
| C17 | Putamen | 1.7 ± 0.5 | 5.0 ± 4.2 | 4.9 ± 2.4 | 1.2 ± 0.6 |
| C29 | Middle temporal gyrus | 1.8 ± 0.4 | 3.2 ± 2.0 | 6.8 ± 3.1 | 2.9 ± 3.0 |
| C30 | Middle temporal gyrus | 1.4 ± 0.3 | 2.6 ± 0.9 | 4.8 ± 2.1 | 3.2 ± 1.6 |
| PiD2 | Middle frontal gyrus | 2.0 ± 0.6 | 31 ± 15 | 15 ± 5.9 | 7.8 ± 4.4 |
| PiD3 | Angular gyrus | 3.1 ± 1.2 | 26 ± 6.1 | 9.9 ± 2.7 | 25 ± 26 |
| PiD4 | Angular gyrus | 5.5 ± 2.9 | 62 ± 5.0 | 19 ± 8.1 | 61 ± 87 |
| PiD5 | Inferior frontal gyrus | 2.2 ± 1.2 | 33 ± 13 | 15 ± 5.9 | 9.0 ± 3.3 |
| PiD6 | Inferior frontal gyrus | 5.3 ± 2.4 | 73 ± 20 | 19 ± 4.9 | 8.0 ± 14 |
| PiD7 | Inferior frontal gyrus | 3.5 ± 1.1 | 25 ± 11 | 11 ± 2.5 | 13 ± 13 |
| sAD11 | Middle temporal gyrus | 9.7 ± 5.2 | 3.0 ± 1.2 | 9.7 ± 1.3 | 51 ± 45 |
| sAD12 | Middle temporal gyrus | 12 ± 4.8 | 3.4 ± 1.6 | 13 ± 3.8 | 38 ± 12 |
| sAD13 | Middle temporal gyrus | 6.4 ± 2.6 | 3.0 ± 1.0 | 19 ± 6.2 | 35 ± 12 |
| sAD14 | Middle temporal gyrus | 13 ± 3.9 | 2.7 ± 0.6 | 12 ± 2.7 | 72 ± 110 |
| sAD15 | Middle temporal gyrus | 9.1 ± 4.8 | 4.0 ± 1.3 | 18 ± 4.4 | 27 ± 16 |
| fAD7 | Middle temporal gyrus | 11 ± 3.4 | 3.2 ± 1.5 | 20 ± 4.3 | 24 ± 17 |
| fAD16 | Middle temporal gyrus | 5.1 ± 3.5 | 1.6 ± 0.6 | 14 ± 6.9 | 12 ± 7.1 |
| CTE1 | Temporal pole | 5.2 ± 2.6 | 13 ± 4.4 | 17 ± 6.4 | 12 ± 7.0 |
| CTE1 | Frontal pole | n.d. | n.d. | n.d. | 11 ± 4.8 |
| CTE2 | Temporal pole | 5.7 ± 4.7 | 9.9 ± 4.3 | 48 ± 32 | 26 ± 16 |
| CTE2 | Frontal pole | n.d. | n.d. | n.d. | 2.9 ± 3.3 |
| CTE3 | Temporal pole | 7.9 ± 4.4 | 6.9 ± 5.9 | 58 ± 35 | 79 ± 56 |
| CTE3 | Frontal pole | n.d. | n.d. | n.d. | 13 ± 6.6 |
| CTE4 | Superior parietal cortex | 10 ± 5.1 | 6.0 ± 1.4 | 79 ± 22 | 90 ± 59 |
| CTE5 | Frontal pole | 4.1 ± 1.8 | 9.3 ± 4.8 | 30 ± 15 | 41 ± 10 |
| AGD1 | Middle frontal gyrus | 15 ± 7.7 | 2.5 ± 1.0 | 27 ± 7.0 | 47 ± 16 |
| AGD2 | Middle insula | 29 ± 21 | 1.2 ± 0.6 | 23 ± 4.4 | 240 ± 110 |
| CBD1 | Angular gyrus | 54 ± 16 | 4.7 ± 1.7 | 26 ± 8.2 | 24 ± 17 |
| CBD2 | Angular gyrus | 42 ± 20 | 2.9 ± 0.84 | 37 ± 11 | 62 ± 10 |
| CBD3 | Angular gyrus | 30 ± 9.0 | 5.0 ± 2.8 | 26 ± 5.6 | 80 ± 26 |
| CBD4 | Angular gyrus | 43 ± 12 | 8.9 ± 3.0 | 47 ± 7.4 | 37 ± 19 |
| CBD5 | Angular gyrus | 27 ± 3.7 | 4.8 ± 3.1 | 26 ± 5.7 | 72 ± 51 |
| PSP1 | Sensorimotor cortex | 67 ± 20 | 13 ± 5.7 | 35 ± 5.6 | 290 ± 16 |
| PSP4 | Inferior frontal gyrus | 86 ± 15 | 4.9 ± 2.2 | 21 ± 4.8 | 110 ± 21 |
| PSP5 | Inferior frontal gyrus | 76 ± 25 | 6.9 ± 2.5 | 40 ± 11 | 94 ± 19 |
| PSP6 | Angular gyrus | 80 ± 13 | 9.1 ± 5.3 | 23 ± 5.1 | 130 ± 31 |
| PSP7 | Inferior frontal gyrus | 110 ± 22 | 13 ± 7.2 | 32 ± 9.6 | 120 ± 24 |
| PSP8 | Inferior frontal gyrus | 30 ± 8.5 | 9.1 ± 4.6 | 20 ± 9.1 | 97 ± 101 |
n.d., not determined; sAD, sporadic AD.
†
Measurements were made from five images per well, n = 6 wells.
‡
Phosphotungstic acid-precipitated samples were diluted in DPBS 1:40 [Tau(4RD*LM)–YFP(1) cells], 1:10 [Tau(3RD*VM)–YFP cells], or 1:4 [Tau(3RD*VM,4R*LM)–YFP cells] before testing.
§
Crude brain homogenate was diluted 1:40 in DPBS before incubation with Tau(4RD*LM)–YFP(2) cells.