Figure 4. Small molecule inhibitors of eIF4A reduce MYC protein levels and suppress cancer cell proliferation.
A. SW480 cells were treated with BEZ235 (200nM) and the indicated concentrations of silvestrol for 48h and analyzed by immunoblotting (n=3).
B. SW480 cells were incubated with BEZ235 (200nM), Silvestrol (25nM) or both. RNA was isolated after 48h and subjected to RQ-PCR analysis (n=2).
C. Immunoblots of four colorectal cell lines upon treatment with increasing concentration of silvestrol or solvent control (n=2).
D. Ls174T cells incubated with increasing concentration of silvestrol for 48h were subjected to RQ-PCR and analyzed for markers of cell cycle arrest (CDKN1A) and differentiation (MUC2) (n=2).
E. Colony formation assay stained with crystal violet. The indicated cell lines were incubated with silvestrol (25nM) for 5 days.