TABLE 1.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′ to 3′)a | Purpose |
|---|---|---|
| BB0647FWD | ATGAACGACAACATAATAGACG | PCR primer |
| BB0647REV | TAAAGTGATTTCCTTGTTCTCATC | PCR primer |
| FLAF | GGTATAATCATATGAACGACAACATAATAGAC | PCR primer |
| FRR | ATGACTCGAGTTATATTCATAAAGTGAT | PCR primer |
| U-19 mer | GTTTTCCCAGTCACGACGT | PCR primerb |
| T7 promoter | TAATACGACTCACTATAGGG | PCR primer |
| GBBEG25F | CAAACTTGGAGAAAACTGG | PCR primer |
| GBBEG25R | CGAACATAAAGTGATTTCCTTG | PCR primer |
| GBBDA28F | TATGAAAATAAATAAATAATAAGTAG | PCR primer |
| GBBDA28R | CGAACATATGATTATACCTTTTTTG | PCR primer |
| GBBBM12F | TTATGCATTGTACTTAAATTGC | PCR primer |
| GBBBM12R | CGAACATAACTATCTCCTTTA | PCR primer |
| FurF | GGGGATAATGATAATCATTATCGGG | Fur box sequencec |
| FurR | CCCGATAATGATTATCATTATCCCC | Fur box sequence |
| PerRF2 | GGGCTAAATTATAATTATTATAATTTAGGGG | Per box sequence |
| PerRR2 | CCCCTAAATTATAATAATTATAATTTAGCCC | Per box sequence |
| LMFv2 | GTATGCGTTGGTACCGCTGCTGGGGGC | Control sequence |
| LMRv2 | GCCCCCAGCAGCGGTACCAACGCATAC | Control sequence |
a
Restriction sites for subsequent cloning of the PCR products are underlined.
b
The U-19 mer and T7 promoter primers were from Novagen.
c
The oligonucleotides used for the Fur box sequence were from Gonzalez de Peredo et al. (25).