FIG. 7.
Binding of Bb-Fur to sites upstream of BB0690 (napA). (A) Target DNA was assayed at 2.4 nM with Bb-Fur at the following concentrations: 0, 6, 12, 23, 59, 117, and 234 nM. The target DNA consisted of the upstream sequence of BB0690 plus flanking vector DNA (BB0690up). (B) Two target DNAs (BsperR and BB0690up) were assayed at 2.4 nM with Bb-Fur at 0 or 23 nM. (C and D) BB0690up DNA was assayed at 2.4 nM with Bb-Fur at 0 or 23 nM. Competitor oligonucleotide (Per box oligo or control oligo) was added to the incubations at 240 nM, as indicated. (E) BsperR DNA was assayed at 2.4 nM with Bb-Fur at 0 or 20 nM. Competitor oligonucleotide (Per box oligo or control oligo) was added to the incubations at 240 nM, as indicated. Bands that are not readily apparent are marked by asterisks.