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Transcription of the fur gene in B. burgdorferi B31-MI. RNA was isolated from spirochetes grown at 23 or 35°C, and the cDNA was synthesized by genome-directed primers. fur was PCR amplified from the cDNA or genomic DNA by fur-specific primers. To control for possible contamination with genomic DNA, the cDNA was prepared with or without RT. DNA size standards (kilobases) appear on the left.
