TABLE 2.
Oligonucleotides used for the site-directed mutagenesis of C. jejuni virB10
| Name | Sequence |
|---|---|
| 5′ virB10 N32A | 5′ GCAGAAGATATATTTGATCAAACAAGCGAAGAAGCTGTATCTAAAAATATATCTAAAAAAGACAATCAAAGC 3′ |
| 3′ virB10 N32A | 5′ GCTTTGATTGTCTTTTTTAGATATATTTTTAGATACAGCTTCTTCGCTTGTTTGATCAAATATATCTTCTGC 3′ |
| 5′ virB10 N42A | 5′ CGAAGAAAATGTATCTAAAAATATATCTAAAAAAGACGCTCAAAGCCAAAATTTGCTTAACAAAGATTTAG 3′ |
| 3′ virB10 N42A | 5′ CTAAATCTTTGTTAAGCAAATTTTGGCTTTGAGCGTCTTTTTTAGATATATTTTTAGATACATTTTCTTCG 3′ |
| 5′ virB10 N97A | 5′ CATAGTGAAGAAAAACCTAAAAAAGAAGAAGATAATGCTATTACTAAGTTAGCAAAAATTGAAGAAAAAAAGCAAGAAC 3′ |
| 3′ virB10 N97A | 5′ GTTCTTGCTTTTTTTCTTCAATTTTTGCTAACTTAGTAATAGCATTATCTTCTTCTTTTTTAGGTTTTTCTTCACTATG 3′ |
| 5′ virB10 N126A | 5′ CAGCAAATTGCAAAAGAAATTCATCAAGATGCTATTAGTTCTCAAGAAAGAAAAATC 3′ |
| 3′ virB10 N126A | 5′ GATTTTTCTTTCTTGAGAACTAATAGCATCTTGATGAATTTCTTTTGCAATTTGCTG 3′ |
| 5′ virB10 N156A | 5′ CAACACGCAAATTTATTTTCAAGAAGCTTCAAAATACGGCGTTGATGGTTTTTC 3′ |
| 3′ virB10 N156A | 5′ GAAAAACCATCAACGCCGTATTTTGAAGCTTCTTGAAAATAAATTTGCGTGTTG 3′ |