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VirB10 possesses affinity for SBA. Neutralized glycine extracts of DB179 were subjected to column chromatography with a SBA-agarose column. Extract, flowthrough, wash, and elution fractions were subjected to SDS-PAGE, blotted onto membranes, and incubated with either anti-VirB8 (A), anti-VirB9 (B), or anti-VirB10 antisera (C) at a 1:50,000 dilution. Lane 1, glycine extract from DB179; lane 2, column flowthrough fraction; lane 3, wash fraction; lanes 4 to 8, elution fractions.
