Figure 3.
CCR2 deficiency reduced endomysial fibrosis of quadriceps and diaphragm muscles at 14 wk but not 6 mo. A) Collagen III immunostaining of mdx5cv and mdx5cv-Ccr2−/− quadriceps (Qua) and diaphragm (Dia) muscles at 14 wk. B) Collagen III immunostaining of mdx5cv and mdx5cv-Ccr2−/− in Qua and Dia muscles at 6 mo. C) Hydroxyproline assay of mdx5cv and mdx5cv-Ccr2−/− in Qua and Dia muscles at 14 wk. D) Hydroxyproline assay of mdx5cv and mdx5cv-Ccr2−/− in Qua and Dia muscles at 6 mo. E) Quantification and comparisons of intramuscular fibroblast numbers in mdx5cv and mdx5cv-Ccr2−/− Qua and Dia muscles at 14 wk. Scale bars, 50 μm. *P < 0.05; **P < 0.01.
