Figure 1.
The ArfGAP Glo3, but not Gcs1, coimmunoprecipitates with the coatomer complex. (A) Protein extracts from a wild-type yeast strain were incubated with anti-Sec21 antibody, followed by incubation with protein A-agarose beads. Beads were pelleted by centrifugation, washed, and resuspended in 1× Laemmli buffer. Samples were run on a 10% SDS-PAGE, transferred to polyvinylidene difluoride membrane, and analyzed with antibodies against Gcs1 and Glo3. (B) The coimmunoprecipitation experiment was repeated in strains lacking Glo3p. The samples were analyzed as in A with antibodies against Gcs1. WCE, whole cell extract.