Fig. 2.
Generation and analysis of Nav1.7 floxed mice. (A) Structure of the native Nav1.7 allele, Nav1.7 targeting construct, fNav1.7 allele, fNav1.7 allele after excision of neor, and Nav1.7 knockout allele. (B) Southern blotting with EcoRI and the external probe confirms correct targeting. (C) Southern blotting with ApaI and internal probe confirms the removal of neor cassette. (D) Southern blotting confirms the deletion of exons 14 and 15 in Nav1.7-/-. (E) PCR was used to detect exon 14-15 deletion in genomic and cDNA from DRG but not spinal cord in Nav1.7R-/-.