FIG. 3.
Entry of cytoplasmatic proteins in the nucleus after poliovirus infection. Mock-infected (a) and poliovirus-infected (b) Hep-2 cells were stained at 3 h p.i. with monoclonal anti-GAPDH antibodies. The secondary antibodies were Cy3 conjugated. The samples were analyzed with a Zeiss Axiovert 100 confocal microscope. HeLa cells were transfected with a plasmid expressing cyclin B-EGFP and 24 h posttransfection were mock infected (c) or poliovirus infected (d) and inspected under epifluorescence microscope at 2 h p.i. 293 cells were transiently transfected with a plasmid expressing five in-frame copies of EGFP and at 24 h posttransfection were infected with poliovirus. The mock-infected (e) and virus-infected (f) cells were investigated under a fluorescence microscope at 2 h p.i.