Table 1.
Effect of inhibition of tri-snRNP formation on concentration of U4/U6-specific and U5-specific components in Cajal bodies
| Cajal bodies/nucleoplasm ratioa±s.d.b (nc) |
|||
|---|---|---|---|
| Protein or snRNA | Control | ΔhPrp31 | ΔhPrp6 |
| hPrp4 | 4.1±0.4 (23) | 13.6±3.6 (23) | 19.8±7.3 (15) |
| hPrp3 | 3.0±0.4 (23) | 12.4±3.8 (24) | 10.6±2.1 (23) |
| p110 | 4.3±1.1 (23) | 11.6±3.5 (24) | 11.1±2.6 (23) |
| U4 snRNA | 3.6±0.4 (24) | 14.0±5.8 (26) | 13.0±6.9 (25) |
| U6 snRNA | 3.4±0.2 (23) | 19.0±5.4 (23) | 21.6±5.7 (24) |
| hSnu114 | 1.4±0.2 (37) | 1.5±0.2 (31) | 1.2±0.1 (29) |
| U5 snRNA | 3.6±0.7 (37) | 4.7±1.5 (26) | 5.1±2.1 (23) |
| U1 snRNA | 2.7±0.3 (23) | 2.8±0.2 (23) | 2.8±0.2 (23) |
| aRatio of average per-pixel fluorescence intensity in the Cajal bodies and nucleoplasm. | |||
| bStandard deviation of random sample of cells (all the photographs in the experiment were used, without selection). | |||
| cn=number of cells evaluated (with an average of 2–3 Cajal bodies per cell). | |||