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. Author manuscript; available in PMC: 2017 Apr 26.
Published in final edited form as: Nature. 2016 Oct 26;540(7632):236–241. doi: 10.1038/nature20165

Figure 5. S-2HG alters global H3K27me3 in CD8+ T-lymphocytes.

Figure 5

a/Immunoblot analysis for H3K27me2 and H3K27me3 in cells treated with S-2HG.

b/ H3K27me3 in OT-I cells treated with S-2HG. n=6 mice.

c/ H3K27me3 abundance in Hif1αfl/fl (n=4) and Hif1αfl/fl dlckcre (n=4) cells treated with S-2HG.

d/ CD62L expression by cells with shUtx #3.

e/In vivo H3K27me3 levels in CD8+ populations. n=6 mice.

f/ ChIP-qPCR for H3K4me3, H3K27me and RNA pol II around the TSS for CD62L. A pool of n=6 mice was used. Error bars denote s.e.m.

One-way ANOVA (b, c). Error bars denote s.d. and each dot in c and e represents a mouse. ns=non-significant, *p<0.05, **p<0.01, ***p<0.001. gMFI=geometric mean fluorescence intensity. For immunoblot source images, see Supplementary Fig. 1.