Table 3. Normalized peak areas for the tryptic peptides of 16 ALDH isozymes.
| ALDH isozymes | Peptide sequence (Q1) | Fragment ion (Q3) | Representative peptide | A549 | EKVK | H322M | H23 | H460 | Hop62 | Hop92 | IMB90 | Primary |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ALDH1A1 | ANNTFYGLSAGVFTK | y10 | N | 2 299 577.2 | 115 108.5 | N.D. | N.D. | 389 508.8 | N.D. | N.D. | N.D. | N.D. |
| ALDH1A1 | LYSNAYLNDLAGCIK | y9 | N | 185 743.7 | N.D.a | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
| ALDH1A1 | TIPIDGNFFTYTR | y11 (2+) | Y | 19 672 834.4 | 1 106 049.4 | N.D. | N.D. | 3 193 847.9 | N.D. | N.D. | N.D. | 12 808.3 |
| ALDH1A3 | ELGEYALAEYTEVK | y7 | Y | N.D. | N.D. | 54 923.5 | N.D. | N.D. | 160 067.6 | N.D. | N.D. | N.D. |
| ALDH1B1 | ELGEYGFHEYTEVK | y10 (2+) | Y | 3 641 491.2 | 171 602.5 | N.D. | N.D. | 744 216.5 | N.D. | N.D. | N.D. | N.D. |
| ALDH1L1 | VLEVEDSTDFFK | y7 | Y | 210 455.5 | 182 277.4 | 72 664.4 | 45 930.7 | 28 234.8 | N.D. | N.D. | N.D. | N.D. |
| ALDH1L2 | ANSTEYGLASGVFTR | y9 | Y | 64 268.2 | 26 451.2 | N.D. | N.D. | 83 066.2 | 46 502.7 | N.D. | N.D. | N.D. |
| ALDH2 | ANNSTYGLAAAVFTK | y9 | N | 187 727.6 | N.D. | N.D. | N.D. | 197 033.4 | 50 184.2 | N.D. | N.D. | 81 064.3 |
| ALDH2 | ELGEYGLQAYTEVK | y9 | N | 150 243.8 | N.D. | N.D. | N.D. | 166 698.5 | 41 966.2 | 46 113.5 | 21 020.0 | 63 511.1 |
| ALDH2 | TIPIDGDFFSYTR | y11 (2+) | Y | 870 545.0 | N.D. | N.D. | 27 259.6 | 908 994.7 | 241 858.7 | 81 402.2 | 58 593.9 | N.D. |
| ALDH3A1 | FDHILYTGSTGVGK | y10 | N | 262 594.5 | N.D. | 62 883.0 | N.D. | 418 238.9 | N.D. | N.D. | N.D. | 7230.6 |
| ALDH3A1 | IQQLEALQR | y7 | Y | 3 587 171.9 | N.D. | 862 497.1 | N.D. | 458 8628.0 | N.D. | N.D. | N.D. | N.D. |
| ALDH3A1 | SLEEAIQFINQR | y6 | N | 1377878.2 | N.D. | 323 073.4 | N.D. | 2 035 663.8 | N.D. | N.D. | N.D. | N.D. |
| ALDH3A2 | IAFGGETDEATR | y9 | N | 561 081.9 | 57 309.1 | 116 4782.8 | 79 575.9 | 207 168.3 | 144 229.1 | N.D. | N.D. | 54 731.4 |
| ALDH3A2 | ILSLLEGQK | y7 | Y | 942 509.0 | 186 310.6 | 120 1969.0 | 209 089.2 | 335 479.5 | 168 177.9 | 45 032.4 | 132 199.9 | 180 608.7 |
| ALDH3A2 | YIAPTVLTDVDPK | y10 (2+) | N | 386 319.7 | 39 480.3 | 714 527.4 | 69 454.7 | 126 169.9 | 86 059.2 | 298 737.1 | N.D. | 121 247.8 |
| ALDH3B1b | FYGDDPQSSPNLGR | y9 | Y | 65 565.9 | 61 949.6 | 27 169.8 | N.D. | 35 658.5 | 8255.1 | N.D. | N.D. | N.D. |
| ALDH4A1 | SSPSLTILAGGK | y5 | Y | 231 740.7 | 275 607.1 | N.D. | 77 972.9 | 149 606.3 | 142 462.3 | 113 616.3 | N.D. | N.D. |
| ALDH5A1 | LAGLSAALLR | y6 | Y | 340 67.6 | 20 330.2 | N.D. | 19 581.7 | 20 374.6 | 13 829.2 | N.D. | N.D. | N.D. |
| ALDH5A1 | VGNGFEEGTTQGPLINE K | y3 | N | 23639.0 | 16 276.1 | 32 714.6 | N.D. | N.D. | 14 859.9 | N.D. | N.D. | N.D. |
| ALDH6A1b | TLADAEGDVFR | y9 | Y | N.D. | 19 405.4 | N.D. | N.D. | 24 117.1 | 69 238.2 | N.D. | N.D. | N.D. |
| ALDH7A1 | GEVITTYCPANNEPIAR | y9 | Y | 845 067.1 | 641 975.1 | 305 186.4 | N.D. | 99 549.5 | N.D. | 16 614.2 | N.D. | N.D. |
| ALDH9A1 | EVNLAVQNAK | y6 | N | N.D. | N.D. | N.D. | N.D. | 92 854.2 | N.D. | N.D. | N.D. | N.D. |
| ALDH9A1 | IGDPLLEDTR | y7 (2+) | Y | 113 934.0 | 78 063.6 | 135 445.2 | N.D. | 243 143.6 | N.D. | N.D. | N.D. | 137 450.8 |
| ALDH9A1 | VTIEYYSQLK | y6 | N | 58 798.9 | N.D. | 59 821.9 | N.D. | 154 409.4 | 60 882.6 | N.D. | N.D. | 50 156.9 |
| ALDH16A1 | EALLVANGTPR | y6 | Y | 83 096.3 | 83 436.6 | N.D. | N.D. | 164 803.8 | 665 40.7 | 48 868.0 | 954 69.8 | N.D. |
| ALDH18A1 | GPVGLEGLLTTK | y9 | Y | 204 825.7 | 78224.8 | 200 087.1 | 205 250.1 | 177 663.5 | 20 7802.9 | 10 6649.8 | 200 954.6 | 575 240.1 |
| ALDH18A1 | LNSLAIGLR | y5 | N | 152 660.6 | 54 133.0 | 91 117.2 | 105 595.7 | 106 207.5 | N.D. | 77 145.7 | 123 819.1 | 190 850.7 |
Abbreviations: ALDH, aldehyde dehydrogenase; N, no; N.D., not determined; Y, yes.
a
N.D.under the criteria of signal-to-noise (SN) ratio >3, coefficient of variation (CV) <25% and raw peak area >5000.
b
Annotated as mitochondrial proteins.