Figure 2.
Biological activity comparison of TLR4 ligands on cell lines. (a) Human and murine TLR4/MD2 transfected cell lines were used to measure fold NF-κB expression. Both human- and murine-based lines show a trend for SLA being more potent with an earlier rise of NF-κB signal and a close to 500-fold difference in the murine line (experiment was performed independently twice, three replicate points averaged). (b) Mono Mac 6 cells were stimulated with either GLA or SLA in an aqueous formulation. This human cell line mirrored the increased sensitivity to stimulation with SLA by producing cytokines at lower levels than those required for signal using GLA.