TABLE 3.
Three replicate blood feeding experiments demonstrate that deficiency in sfRNA1 and sfRNA2 results in decreased WNV infection and transmission rates in C. pipiensa
| Virus | Replicate | n | Positive bodies (no.) | Positive saliva (no.) | Infection (%) | Transmission (%) | Dissemination (%) |
|---|---|---|---|---|---|---|---|
| WNVIC | 1 | 23 | 8 | 7 | 35 | 30 | 88 |
| 2 | 28 | 3 | 2 | 11 | 7 | 67 | |
| 3 | 22 | 1 | 1 | 5 | 5 | 100 | |
| Total | 73 | 12 | 10 | 16 | 14 | 83 | |
| WNVΔSF1 + 2 | 1 | 18 | 0 | 0 | 0 | 0 | 0 |
| 2 | 36 | 2 | 2 | 6 | 6 | 100 | |
| 3 | 29 | 0 | 0 | 0 | 0 | 0 | |
| Total | 83 | 2 | 2 | 2 | 2 | 100 |
a
Shown are infection, transmission, and dissemination rates of orally infected C. pipiens mosquitoes with either WNVIC or WNVΔSF1+2. In addition, the sample size (n) as the number of engorged females after a blood meal and absolute numbers of positive bodies and saliva samples are shown for each replicate.