TABLE 1.
Four replicate blood-feeding experiments demonstrate that sfRNA determines the infection and transmission rates of WNV in C. pipiens mosquitoesa
| Virus | Replicate | n | Positive bodies (no.) | Positive saliva (no.) | Infection (%) | Transmission (%) | Dissemination (%) |
|---|---|---|---|---|---|---|---|
| WNVIC | 1 | 33 | 11 | 11 | 33 | 33 | 100 |
| 2 | 39 | 20 | 14 | 51 | 36 | 70 | |
| 3 | 24 | 5 | 4 | 21 | 17 | 80 | |
| 4 | 44 | 9 | 8 | 20 | 18 | 89 | |
| Total | 140 | 45 | 37 | 32 | 26 | 82 | |
| WNVΔSF1 | 1 | 37 | 8 | 6 | 22 | 16 | 75 |
| 2 | 40 | 3 | 3 | 8 | 8 | 100 | |
| 3 | 23 | 2 | 2 | 9 | 9 | 100 | |
| 4 | 49 | 6 | 4 | 12 | 8 | 67 | |
| Total | 149 | 19 | 15 | 13 | 10 | 79 |
a
Shown are infection, transmission, and dissemination rates of C. pipiens mosquitoes orally infected with either WNVIC or WNVΔSF1. In addition, the sample size (n) as the number of engorged females after a blood meal and absolute numbers of positive bodies and saliva samples are shown for each replicate.