Figure 5. A1ATm358 inhibits NET formation.
(A) Isolated adult neutrophils were preincubated in medium (1 hour) and then incubated alone (control) or with LPS (100 ng/ml, 1 hour), followed by live cell imaging as in Figure 1A. Original magnification, ×60. Scale bars: 20 μm. Red fluorescence, yellow arrows = NETs. (B) In parallel, neutrophils were preincubated with synthesized A1ATm358 or scrambled A1ATm358 (A1ATm358–SCR) (1, 10, or 100 nM, 1 hour) and activated with LPS; NETs were assessed by live cell imaging after incubation for 1 hour. A second experiment yielded a similar concentration-dependent pattern of inhibition by A1ATm358 but not A1ATm358–SCR. Original magnification, ×60. Scale bars: 20 μm.