Fig. 1.
Longitudinal imaging with PET. (A) Layout of experimental data. Each row of images corresponds to a given animal followed in the study (identified by number at left), and each column corresponds to a time point for imaging. Animals were followed longitudinally from as early as 10 days to as late as 93 days posttransplantation of MIN-O tissue. Four animals were not imaged at the earliest time point because of the lack of a visible signal in the other six animals. At the last time point in each row, animals were killed and the mammary fat pads were prepared for ex vivo imaging and histology. (B) Example image. An enlarged view of a maximum-intensity projection of a study mouse showing uptake in various tissues, including the MIN-O tissue in the left and right no. 4 mammary fat pads. The mouse is displayed in a supine orientation. (C) Intensity scale. The color scale representing uptake relative to brain. This color scale is used for all PET images.