Table 1. Binding of rgHK68 viruses in a glycan arraya.
| Group | Glycan N0. | Sialic acid structure | Sialic acid distribution in human RT |
Intensity of binding (RFUb ± SDc) |
||||
|---|---|---|---|---|---|---|---|---|
| Nasopharynx and tonsil | Bronchus | Lung | rgHK68 | rgHK68 + 2 | rgHK68 + 4 | |||
| I | 57 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp24 | + | + | 1900 ± 191 | 585 ± 33 | 1492 ± 49 | |
| 237 | Neu5Acα2-3GalNAcβ1-GlcNAcβ- Sp0 | 1190 ± 138 | 175 ± 44 | 1420 ± 137 | ||||
| 258 | Neu5Acα2-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcb-Sp0 | + | + | + | 490 ± 56 | 120 ± 10 | 215 ± 34 | |
| 266 | Neu5Acα2-6GalNAcβ1-GlcNAcβ- Sp0 | 1062 ± 342 | 237 ± 101 | 301 ± 54 | ||||
| 271 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ-Sp0 | + | + | + | 1393 ± 71 | 235 ± 30 | 653 ± 184 | |
| 325 | Neu5Acα2-3Galβ1-4GlcNAcβ1-2Mana1-6(Neu5Acα2-3Galβ1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | + | + | + | 1230 ± 125 | 351 ± 8 | 930 ± 109 | |
| 332 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ-Sp0 | + | + | + | 1550 ± 128 | 350 ± 16 | 1740 ± 156 | |
| 464 | Neu5Acα2-6Galβ1-4GlcNAcβ1-4Mana1-6(GlcNAcβ1-4) (Neu5Acα2-6Galβ1-4GlcNAcβ1-4(Neu5Acα2-6Galβ1-4GlcNAcβ1-2)Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp21 | + | 2008 ± 78 | 121 ± 15 | 1020 ± 208 | |||
| 466 | Neu5Acα2-6Galβ1-4GlcNAcβ1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-2)Mana1-6(GlcNAcβ1-4) (Neu5Acα2-6Galβ1-4GlcNAcβ1-4(Neu5Acα2-6Galβ1-4GlcNAcβ1-2)Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp21 | 1817 ± 69 | 150 ± 31 | 975 ± 349 | ||||
| 482 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4(Fuca1-6)GlcNAcβ-Sp24 | + | + | + | 1390 ± 97 | 1147 ± 75 | 1530 ± 40 | |
| 483 | Neu5Acα2-3Galβ1-4GlcNAcβ1-2Mana1-6(Neu5Acα2-3Galβ1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4(Fuca1-6)GlcNAcβ-Sp24 | + | + | + | 1250 ± 46 | 403 ± 32 | 635 ± 82 | |
| II | 55 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | + | + | 433 ± 291 | 50 ± 14 | 358 ± 64 | |
| 236 | Neu5Acα2-3GalNAcα-Sp8 | 688 ± 56 | 39 ± 18 | 129 ± 67 | ||||
| 241 | Neu5Acα2-3Galβ1-4(Neu5Acα2-3Galβ1-3)GlcNAcβ-Sp8 | 350 ± 43 | 13 ± 11 | 121 ± 46 | ||||
| 247 | Neu5Acα2-3Galβ1-3GlcNAcβ1-3Galβ1-4GlcNAcβ-Sp0 | + | + | + | 215 ± 35 | 13 ± 13 | 233 ± 37 | |
| 249 | Neu5Acα2-3Galβ1-3GlcNAcβ-Sp0 | 202 ± 25 | 10 ± 12 | 187 ± 22 | ||||
| 251 | Neu5Acα2-3Galβ1-4(6S)GlcNAcβ-Sp8 | 115 ± 43 | 11 ± 7 | 283 ± 23 | ||||
| 253 | Neu5Acα2-3Galβ1-4(Fuca1-3)GlcNAcβ1-3Galβ1-4(Fuca1-3)GlcNAcβ1-3Galβ1-4(Fuca1-3)GlcNAcβ-Sp0 | 101 ± 16 | 2 ± 8 | 227 ± 33 | ||||
| 270 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galb1-4(Fuca1-3)GlcNAcβ1-3Galβ1-4(Fuca1-3)GlcNAcβ-Sp0 | 120 ± 82 | 99 ± 12 | 1240 ± 159 | ||||
| 318 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-6(Neu5Acα2-3Galβ1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | + | + | 218 ± 110 | 45 ± 12 | 1224 ± 99 | ||
| 320 | GlcNAcβ1-2Mana1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 358 ± 31 | 11 ± 10 | 992 ± 72 | ||||
| 326 | Neu5Acα2-3Galβ1-4GlcNAcβ1-2Mana1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | + | + | 1060 ± 64 | 61 ± 21 | 500 ± 61 | ||
| 345 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-6(Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAc-Sp12 | 144 ± 37 | 7 ± 4 | 360 ± 34 | ||||
| 346 | Mana1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAc-Sp12 | 577 ± 39 | 22 ± 16 | 795 ± 71 | ||||
| 347 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-6Manβ1-4GlcNAcβ1-4GlcNAc-Sp12 | 190 ± 44 | 11 ± 3 | 635 ± 82 | ||||
| 348 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-3Manβ1-4GlcNAcβ1-4GlcNAc-Sp12 | 808 ± 86 | 18 ± 10 | 975 ± 88 | ||||
| 366 | Neu5Acα2-6GlcNAcβ1-4GlcNAc-Sp21 | 663 ± 148 | 10 ± 5 | 2792 ± 246 | ||||
| 367 | Neu5Acα2-6GlcNAcβ1-4GlcNAcβ1- 4GlcNAc-Sp21 | 776 ± 151 | 9 ± 5 | 3325 ± 529 | ||||
| 441 | Neu5Acα2-3Galβ1-4GlcNAcβ1-3 Galβ-Sp8 | 115 ± 43 | 4 ± 4 | 205 ± 10 | ||||
| 462 | Neu5Acα2-3Galβ1-4GlcNAcβ1-6(Neu5Acα2-3Galb1-4GlcNAcβ1-2)Mana1-6(GlcNAcβ1-4)(Neu5Acα2-3Galβ1-4GlcNAcβ1-4(Neu5Acα2-3Galβ1-4GlcNAcβ1-2)Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp21 | 853 ± 136 | 20 ± 12 | 187 ± 39 | ||||
| 463 | Neu5Acα2-6Galβ1-4GlcNAcβ12 Mana1-6(GlcNAcβ1-4)(Neu5 Acα2-6Galβ1-4GlcNAcβ1-2Mana1-3)Manb1-4GlcNAcβ1-4GlcNAcβ-Sp21 | 345 ± 97 | 6 ± 7 | 1692 ± 156 | ||||
| 465 | Neu5Acα2-6Galβ1-4GlcNAcβ1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-2)Mana1-6(GlcNAcβ1-4) (Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp21 | + | 432 ± 93 | 5 ± 2 | 1600 ± 89 | |||
| 481 | Neu5Acα2-6Galβ1-4 GlcNAcβ1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-3)GalNAcα-Sp14 | 1667 ± 114 | 52 ± 31 | 2008 ± 220 | ||||
| 601 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-6(Galβ1-3) GalNAcα-Sp14 | 830 ± 149 | 49 ± 12 | 1023 ± 104 | ||||
| III | 56 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Man-a1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp21 | + | + | 355 ± 50 | 8 ± 4 | 79 ± 25 | |
| 250 | Neu5Acα2-3Galβ1-3GlcNAcβ-Sp8 | 296 ± 50 | 6 ± 5 | 41 ± 12 | ||||
| 259 | Neu5Acα2-3Galβ1-4GlcNAcβ-Sp0 | 205 ± 77 | 15 ± 13 | 54 ± 16 | ||||
| 260 | Neu5Acα2-3Galβ1-4GlcNAcβ-Sp8 | 208 ± 47 | 10 ± 4 | 89 ± 19 | ||||
| 261 | Neu5Acα2-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ-Sp0 | + | + | + | 138 ± 27 | 44 ± 32 | 95 ± 24 | |
| 264 | Neu5Acα2-3Galβ1-4Glcβ-Sp8 | 180 ± 40 | 13 ± 4 | 31 ± 13 | ||||
| 267 | Neu5Acα2-6Galβ1-4(6S)GlcNAcβ-Sp8 | 510 ± 26 | 20 ± 13 | 99 ± 27 | ||||
| 268 | Neu5Acα2-6Galβ1-4GlcNAcβ-Sp0 | 378 ± 271 | 8 ± 10 | 47 ± 27 | ||||
| 269 | Neu5Acα2-6Galβ1-4GlcNAcβ-Sp8 | 983 ± 123 | 31 ± 9 | 99 ± 79 | ||||
| 308 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-6(GlcNAcβ1-2Mana1-3)Manb1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 350 ± 43 | 9 ± 5 | 60 ± 49 | ||||
| 319 | Galβ1-4GlcNAcβ1-2Mana1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | + | + | + | 490 ± 75 | 14 ± 7 | 62 ± 31 | |
| 330 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3 Galβ1-3GlcNAcβ-Sp0 | 727 ± 146 | 1 ± 3 | 77 ± 41 | ||||
| 479 | Neu5Acα2-3Galβ1-4GlcNAcβ1-6 GalNAcα-Sp14 | 185 ± 37 | 8 ± 4 | 27 ± 16 | ||||
| 491 | Neu5Acα2-3Galβ1-3GlcNAcβ1-6 GalNAcα-Sp14 | 300 ± 50 | 99 ± 11 | 18 ± 21 | ||||
| 502 | Neu5Acα2-6GalNAcβ1-4(6S) GlcNAcβ-Sp8 | 730 ± 91 | 14 ± 7 | 79 ± 42 | ||||
| 597 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3GalNAcα-Sp14 | 459 ± 84 | 32 ± 20 | 41 ± 19 | ||||
| 600 | Neu5Acα2-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-6(Galβ1-3) GalNAcα-Sp14 | 343 ± 58 | 45 ± 40 | 90 ± 37 | ||||
| 602 | Neu5Acα2-6Galβ1-4GlcNAcβ1-6 (Galβ1-3)GalNAcα-Sp14 | 880 ± 14 | 111 ± 75 | 39 ± 20 | ||||
| 605 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4 GlcNAcβ1-3)GalNAcα-Sp14 | 512 ± 90 | 18 ± 11 | 13 ± 11 | ||||
| 606 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-2Mana1-6(Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4 GlcNAcβ1-3Gaβb1-4GlcNAcβ1-2Mana1-3)Manβ1-4GlcNAcβ1-4 GlcNAcβ-Sp12 | 1325 ± 368 | 12 ± 21 | 99 ± 104 | ||||
| 608 | Neu5Acα2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-2Mana1-6 (Neu5Acα2-6Galβ1-4GlcNAcβ1-3 Galβ1-4GlcNAcβ1-2Mana1-3) Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 385 ± 152 | 1 ± 4 | 70 ± 52 | ||||
| IV | 255 | Neu5Acα2-3Galβ1-4(Fuca1-3) GlcNAcβ-Sp8 | 33 ± 39 | 10 ± 5 | 200 ± 6 | |||
| 295 | Neu5Acα2-3Galβ1-4GlcNAcβ1-3Galβ1-3GlcNAcβ-Sp0 | 36 ± 38 | 5 ± 5 | 227 ± 19 | ||||
| 301 | Neu5Acα2-6Galβ1-4GlcNAcβ1-2 Mana1-6(Galβ1-4GlcNAcβ1-2 Mana1-3)Manβ1-4GlcNAcβ1-4 GlcNAcβ-Sp12 | + | + | + | 93 ± 48 | 16 ± 10 | 450 ± 55 | |
| 459 | Neu5Acα2-3Galβ1-4GlcNAcβ1-2 Mana1-6(GlcNAcβ1-4) (Neu5Acα2-3Galβ1-4GlcNAcβ1-2 Mana1-3)Manβ1-4GlcNAcβ1-4 GlcNAcβ-Sp21 | 16 ± 9 | 6 ± 2 | 610 ± 5 | ||||
| 460 | Neu5Acα2-3Galβ1-4GlcNAcβ1-4 Mana1-6(GlcNAcβ1-4) (Neu5Acα2-3Galβ1-4GlcNAcβ1-4 (Neu5Acα2-3Galβ1-4GlcNAcβ1-2)Mana1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp21 | + | 18 ± 20 | 11 ± 8 | 226 ± 70 | |||
| 461 | Neu5Acα2-3Galβ1-4GlcNAcβ1-6 (Neu5Acα2-3Galβ1-4GlcNAcβ1-2)Mana1-6(GlcNAβb1-4) (Neu5Acα2-3Galβ1-4GlcNAcβ1-2 Mana1-3)Manβ1-4GlcNAcβ1-4 GlcNAcβ-Sp21 | + | 47 ± 41 | 6 ± 6 | 825 ± 23 | |||
aBinding of viruses (screened at three concentrations [Fig. 2C] but all normalized to 10, 200 HAU and averaged) was analyzed on the Consortium for Functional Glycomics printed array v5.1 containing 610 glycans (www.functionalglycomics.org). Human RT-associated SA structures are highlighted. Group I glycans were recognized by all three rgHK68 viruses. Group II SAs were recognized by rgHK68 and rgHK68 + 4 only. Groups III and IV SAs were recognized only by rgHK68 or rgHK68 + 4, respectively. Binding below 100 RFU was regarded as irrelevant.
bRFU–relative fluorescent units.
cSD–standard deviation.