Figure 3. Reconstitution of macrophages in the peritoneum rescues vascular permeability.
A) Schematic experimental design for the clodronate liposome injections. Clod, clodronate; i.p., intraperitoneal injection; Exp, evaluation of permeability. B) Peritoneal macrophages were quantified by flow cytometry using Mrc1 expression after injection of clodronate liposomes (Clod) or PBS-liposome (Ctrl), n=5. Statistics: Wilcoxon rank sum test, p=0.0159. C) Kinetics of permeability were measured by FITC-ovalbumin leakage into peritoneal cavity over time, n=5 for each time point. Statistics: Wilcoxon rank sum test, p=0.0159 (for 10, 30 and 60min), p=0.0286 (for 3hrs). D) Evaluation of permeability after macrophage reconstitution (106 cells per animal) and measured by TRITC-Dextran exit from the blood stream into the peritoneal cavity after 60 minutes. n= 3 for control group, n= 8 for injected macrophages. Statistics: Wilcoxon rank sum test, p=0.0079.