Figure 3.
HLA Class II-Restricted CD8αβ T Cells in Three HIV Controllers Exhibit Heterogeneous Memory Phenotypes
(A) Representative FACS plots denoting surface expression of CD8α and CD8β. Tetramer-positive expression is shown in red. Bulk CD3+CD4− T cells are shown in gray. CD3+CD4+ T cells are shown in black.
(B) Violin plots denoting the distribution of single-cell expression levels of CD8α (CD8A), CD8β (CD8B), and CD4 RNA transcripts in unstimulated class II tetramer-sorted CD8+ and CD4+ T cells estimated from single-cell RNA-seq. Abbreviation: TPM, transcripts per kilobase million. Statistical significance was determined with Mann-Whitney-Wilcoxon test (∗∗∗p < 0.001).
(C) Representative FACS plots show memory phenotype of tetramer-positive HLA class I- and class II-restricted CD8+ T cells. All populations shown are gated on tetramer+ CD3+CD8+CD4−CD19−CD14−CD56− live lymphocyte singlets.
See also Figure S4.