Skip to main content
. 2016 Oct 18;45(4):917–930. doi: 10.1016/j.immuni.2016.09.015

Figure 1.

Figure 1

Elucidation of a HLA Class II DRB1-Restricted CD8+ T Cell Response in HIV Controller 474723

(A) Representative IFN-γ HLA-DR Elispot using magnetically enriched CD8+ T cells (FACS plot insert) co-cultured with an LCL stably transfected with DRB1∗11:01 (matching the HLA type of subject) pulsed with 66 overlapping peptides (OLPs) spanning the HIV Gag protein. Error bar represents SD.

(B) Representative IFN-γ HLA-DR Elispot performed with N- and C-terminal peptide truncations presented on DRB1∗11:01 LCL. Epitope FA10 within Gag41 is highlighted in red.

(C) Representative flow cytometric intracellular cytokine secretion (ICS) assay with anti-HLA-DR antibody performed on whole PBMCs co-cultured with DRB1∗11:01 LCL.

See also Figures S1–S3 and Table S1.