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. 2016 Oct 19;6:35434. doi: 10.1038/srep35434

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Copyright © 2016, The Author(s)

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PHH and HepaRG spheroids were repeatedly exposed to the model cholestatic compound chlorpromazine (CPZ) for 8 days. (A) BA accumulation was assessed by using the fluorescently labelled taurocholic acid derivative tauro-nor-THCA-24-DBD. Note that 10 μM CPZ was toxic to the HepaRG spheroids. (B) Expression of ABCB11, encoding BSEP, was analysed by RT-qPCR and normalized to the expression of the housekeeping gene GAPDH. Data represent the means ± SEM of 3 independent experiments. **p < 0.01. (C) F-actin cytoskeleton integrity was visualized by Phalloidin staining. All scale bars = 100 μm.