Figure 7.
Regulation of FTH1 expression during ferroptosis. (A) Ferroptosis induced an increase of FTH1 protein levels. HT1080 cells were treated with 5 μM erastin for the indicated times. Total cell lysate was used to detect FTH1 expression. (B) Ferroptosis induced an upreguation of FTH1 mRNA levels. HT1080 cells were treated with 5 μM erastin as indicated. Total RNA was isolated and mRNA level of FTH1 was measured by q-PCR. (C) NCOA4 is required for ferroptosis-induced FTH1 mRNA upregulatioin. HT1080 cells with or without NCOA4 depletion were treated with 5 μM erastin for 10 h. Total RNA was isolated and mRNA level of FTH1 was measured by q-PCR. (D) BafA1 inhibits ferroptosis-associated FTH1 degradation. HT1080 cells were treated as indicated. Total cell lysate was used for immunoblotting to detect the changes of target protein levels.