Figure 6. Loss of Rb results in increased expression of proteins involved in metastasis and neuroendocrine differentiation in prostate cancer cells in a hypoxia-dependent fashion.
(A) LNCaP and (B) 22Rv1 shRNA cells were exposed to normal O2 levels or 1% O2 for various times up to 7 days as indicated. Whole cell lysates were fractionated by SDS-PAGE and examined by immunoblotting using affinity purified antibodies to α–tubulin, Rb, AR, ENO2, KISS1R, RORα, PLOD2, NDRG1 and HTR5A.