Figure 4. Rb-loss leads to transcriptional dysregulation of hypoxia-regulated genes involved in metastasis, angiogenesis and neuroendocrine differentiation.
(A) Heat map of the 50 genes displaying the largest increase in fold gene expression in response to hypoxia after knock-down of Rb. (B) The top systems and diseases identified by Ingenuity Pathway Assist (IPA) analysis. The top 12 systems and diseases associated with the 50 most up-regulated and 50 most down-regulated hypoxia sensitive genes after knock-down of Rb. The –log of the p-value is represented on the ordinate. (C) Nervous system development and function network identified by IPA analysis. Nodes identified are indicated with red circles. Up-regulated genes identified in our micro-array screen are filled with varying shades of pink and red with the most highly expressing genes shaded red and the lower expressing genes shaded pink.