Extended Figure 7. Translating ribosome affinity purification (TRAP) after cancer cell astrocyte co-culture.
a, Schematic illustration of TRAP experimental set up to isolate translating mRNA from MDA231-BrM2 cells under 3 conditions (#1, #2, #3). b, Principle component (PC) analysis of TRAP mRNA sequencing. c. Scatter plot of log2 fold-changes regulated by astrocytes and gap junction communications between BrM cells and astrocytes. d, STAT1 and NF-κB p65 phosphorylation in H2030-BrM3 cells after a 2 h incubation with conditioned media (CM) from astrocyte co-cultures. CM were collected after 24 h co-culture of astrocytes with control or Cx43-depleted H2030-BrM3 cells. n=3 independent experiments.