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(A) The crystal structure of tyrosine binding site of the wild-type mjYRS (based on Protein Data Bank structure 1J1U). (B) The aminoacylation assay [13] of wild-type mjYRS for tyrosine and its variants for pTyr. 10 µM wild-type mjTyrRS or 100 µM mjTyrRS variants and 1 mM tyrosine or 10 mM pTyr were used in the assay. The mean values and standard errors were calculated from three replicates.
