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. 2016 Jul 5;5(7):e330. doi: 10.1038/mtna.2016.45

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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LK-L1C/K6W/L8C promotes in vitro Dicer processing of precursor-microRNA-29b (pre-miR29b), but not that of pre-miR29a. (a) In vitro processing assay for pre-miR29b was performed by treating the indicated peptides with immunopurified human Dicer. The dicing reaction was conducted for 2.5 minutes. The RNA size markers (decade marker, Ambion) were displayed on the left side of the gel. Values indicate ratios of mature miRNA to mature and precursor miRNA was quantified and normalized with mock sample. Relative levels of mature miR29b were presented below (n = 3, mean ± SD). (b) In vitro Dicer processing of pre-miR29a was carried out with peptides. Proportion of mature miRNA was quantified and normalized, as described in Figure 2a. Relative levels of mature miR29a were indicated below (n = 3, mean ± SD).