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. 2016 Jul 14;35(16):1766–1778. doi: 10.15252/embj.201694696

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© 2016 The Authors. Published under the terms of the CC BY NC ND 4.0 license

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs 4.0 License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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A–C
Cryo‐electron micrographs of GSDMD^Nterm pores in E. coli polar lipid liposomes. The micrographs were acquired at protein/lipid molar ratios of 1/1,000, 1/500, and 1/100, respectively. Black arrows indicate ring‐shaped structures corresponding to oligomeric GSDMD^Nterm pore forms. Scale bars = 80 nm.

D
Proteoliposome with protein/lipid molar ratio of 1/100 at higher magnification. Black arrows indicate ring‐shaped structures corresponding to oligomeric GSDMD^Nterm pore forms. Scale bar = 80 nm.