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. Author manuscript; available in PMC: 2017 Aug 19.
Published in final edited form as: Circ Res. 2016 Jun 30;119(5):635–651. doi: 10.1161/CIRCRESAHA.116.308937

Figure 7. Analysis of myocyte cross-sectional area and myocyte density.

Figure 7

Myocyte cross-sectional area and myocyte density were determined in rat hearts stained with WGA (green) and α-SA (red). A. Representative epifluorescent microscopic image, sequentially acquired from 18 fields of the infarcted region of a vehicle-treated rat at a magnification of x300. Myocytes with round nuclei and clearly defined sarcolemmal borders were selected for analysis of cross-section area. B and C. Quantitative analyses of myocyte cross-sectional area (B) and myocyte density (C) per mm2. WGA binds to the myocyte membrane, thereby facilitating the evaluation of myocyte cross-sectional area and density. The risk region comprises both the border zones and the infarcted region. Data are means ± SEM. Bar is 100 µm.