Figure 5. NR reverses deficits in muscle mass, strength, and exercise capacity of mNKO mice.
A, Treadmill performance of 5.5-month-old mice before beginning NR administration (n=5–6).
B, Treadmill performance after 1 week of treatment and
C, Change in exhaustion time of individual mice at the point of exhaustion across the first week of treatment (n=5–6).
D, Treadmill performance after 5 weeks of NR treatment (n=5).
E, Mass of hindlimb muscles isolated after 6 weeks of NR administration (n=5–6).
F, Twitch force and
G, Tetanic force generated by isolated EDL muscle. Dashed lines indicated error bars. Scale bars indicate 100 msec. Insets: maximum force generated per subject (n=5–6).
H, Intramuscular NAD content and
I, Intramuscular ATP content assessed in quadriceps after 6 weeks of treatment (n=5–6). Error bars represented SEM. Significance was determined by one-way ANOVA with Tukey’s post-hoc test (ns, not significant, *p<0.05,***p<0.001). Mice were male aged 7 months at sacrifice. See also Figures S5 and S6.