Fig. 3.
Pathway analysis reveals that most highly regulated inflammatory mediators are associated with the TREM-1 signaling pathway. The preprogrammed TREM-1 signaling pathway was merged with the preprogrammed genes known to be highly modulated during esophagitis (Ingenuity Systems 2014). This dataset was overlaid with the real gene expression array data obtained from the esophageal tissue of the different experimental groups to identify modulated genes. The red color indicates the upregulation of a particular gene expression, and the green color indicates downregulation. The intensity of the color signifies the magnitude of regulation. Grey color indicates no gene modulation. For a better overview, genes that were not modulated in any of the groups were removed from the pathway. The figure shows gene expression of the esophagitis group compared with the sham-operated group (A), the STW5- treated (0.5 and 2 mL/kg, respectively) groups in comparison to the esophagitis group (B, C) and the omeprazole-treated (30 mg/kg) group in comparison to the esophagitis group (D).