Figure 4.
Histone H3 hyper Ac is induced by LPS in the area spanning the Iγ1–Cγ1 locus. Anti-H3Ac histone antisera were used in ChIP assays carried out on nuclei derived from splenic B cells. Cells were activated with LPS or with LPS + IL–4 for 48 h or were unstimulated. A representative experiment is shown using three independent ChIP samples. All quantitative PCR values were normalized against input DNA to obtain the Ac histone index. Averaged histone Ac indices and SD downstream of the Sμ region (Sμ-D) and Sγ1 (Sγ1-D) are shown.