FIGURE 7.
Ly6C+ CD4 T cells are reduced in the ependyma (EP) but not trigeminal ganglia (TG) or brain stem (BS) by 60 DPI. (A–C) CD4+ TRM (CD3+ CD4+ CD44+ CD103+ CD69+) cells identified by flow cytometry at 60 DPI in the BS and EP compared to the TG. TRegs (CD4+ CD25+ FoxP3+) in the TG (D), BS (E) and EP (F) at 30–60 DPI. (G–I) Th1-type cells (CD4+ Ly6C+) at 30–60 DPI in the TG (G), BS (H), and EP (I) in comparison to uninfected (Ui) controls. The bars represent the mean + SEM and summarizes 2–3 independent experiments per time point with Ui n=6, 30 DPI n=7, and 60 DPI n=9/group. *p<0.05, **p<0.005 comparing the indicated groups as determined by Kruskal-Wallis ANOVA