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. Author manuscript; available in PMC: 2016 Aug 3.
Published in final edited form as: Nat Immunol. 2013 Jun 16;14(8):785–792. doi: 10.1038/ni.2631

Figure 1. Platelets form “touch-and-go” interactions within the liver sinusoids.

Figure 1

(a) Representative intravital microscopy images of wild-type mouse liver. Platelets labeled with PE-conjugated anti-CD49b (red); Kupffer cells labeled with Alexa Fluor 647-conjugated anti-F4/80 (blue). Yellow arrows denote rapid and transient “touch-and-go” behavior of platelets on KC. (b) Quantification of the number of platelet “touch-and-go” interactions with KC or the endothelium within the livers of wild-type, KC-depleted (KC-depl), GpIbα−/− (GPIbα-def) and Cd41-yfpki/ki (CD41-def) mice in basal conditions. n ≥ 3 individual mice per group; error bars, SEM; *P < 0.01 by one-way ANOVA. (c) Quantification of the total number of platelet “touch-and-go” interactions within the livers of wild-type, KC-depleted, GpIbα−/− (GPIbα-def) and Cd41-yfpki/ki (CD41-def) mice in basal conditions. n ≥ 3 individual mice per group; error bars, SEM; *P < 0.01; **P < 0.001 by one-way ANOVA. (d) Quantification of the total number of platelet “touch-and-go” interactions within the livers, brain, muscle, skin and ear of wild-type in basal conditions. n ≥ 3 individual mice per group; error bars, SEM; *P < 0.001 vs Liver, one-way ANOVA. See also Supplementary Video 1.