Figure 3. Recipient cDCs that acquire intact donor MHC molecules in vivo promote T cell immunity.
(A) Recipient cDCs carrying donor MHC (FACS-sorted in R1 of Figure 2A) triggered proliferation of 2C CD8 T cells against BALB/c H2Ld. (B and C) Expression of T cell activation/effector markers (B) and cytokine secretion (C) of 2C T cells following stimulation by recipient (B6) splenic cDCs carrying donor MHC (FACS-sorted in R1 of Figure 2A), or by control cDCs. Numbers in dot plots indicate percentages of cells in the corresponding quadrants. IL-5 and IL-17A were undetectable by ELISA. In A–C, 1 representative of 4 experiments is shown. P values were generated by 1-way ANOVA followed by Tukey-Kramer multiple comparisons test.