Extended Data Figure 6. AAVR endocytosis is crucial for AAV2 infection.
a, Schematic of the AAVR minimal construct (miniAAVR) and domain-swapped derivatives probing the localization of AAVR through the swapping of AAVR’s C-tail with that of well-characterized recycling receptors: cation-independent mannose-6-phosphate receptor (Ci-MPR) (traffics from plasma membrane (PM) through endosomes to the TGN), low density lipoprotein receptor (LDLR) and poliovirus receptor (PVR) (both traffic from PM to endosomal compartments but are not reported to traffic to TGN). b, Corresponding permeabilized and unpermeabilized immunofluorescence images of constructs depicted in a when expressed in AAVRKO cells. c, AAV2-RFP infection (MOI 20,000 vg/cell; measured after 24 hrs) in AAVRKO cells stably expressing constructs depicted in a. Data depicts the mean with s.d. for triplicate infections. Scale bars represent 10 μm.