Figure 3.
Inhibition of ptena restores mTOR kinase activity in the OPCs of hmgcs1 mutant larvae. Representative images of living 4 dpf larvae are included for each condition. A–D, Representative single optical sections selected from z-stack datasets of 4 dpf Tg(olig2:EGFP) larvae labeled with antibodies to detect pS6 (red) and GFP (green). Insets show only the red channel for outlined areas. A, B, Images of pS6+ OPCs (arrows) in wild-type larva injected with p53 MO alone, as a control, and wild-type larva injected with both p53 and ptena MO. C, Image of pS6− OPC (arrow) in hmgcs1 mutant larva injected with p53 MO. D, pS6+ OPC of an hmgcs1 mutant larva injected with both p53 and ptena MO. E, Graph showing the percentages of dorsal pS6+ OPCs (n = at least 23 larvae for each genotype and treatment; p values were calculated using a Mann–Whitney test). F–I, Representative spinal cord images, viewed from a lateral position, obtained from 4 dpf Tg(olig2:EGFP) larvae labeled with antibodies to detect pErk (red) and GFP (green). F, G, Examples of pErk+ OPCs in a wild-type larva injected with p53 MO and wild-type larva injected with both p53 and ptena MO. H, I, pErk− OPCs in a hmgcs1 mutant larva injected with p53 MO and hmgcs1 mutant larva injected with both p53 and ptena MO. J, Graph showing the percentages of pErk+ dorsal OPCs (n = at least 20 larvae for each genotype and treatment; p values were calculated using a Mann–Whitney test). Error bars represent the SEM. Scale bars, 10 μm.