Figure 3.
Characterization of CCCP-induced cardiolipin externalization in HeLa cells transfected with wild-type or R90D mutant of NDPK-D. (a) HeLa cells were transiently transfected with vectors expressing w/t or R90D NDPK-D for 48 h followed by treatment with 20 μM CCCP for 4 h. Crude mitochondria were isolated for determination of CL externalization using Annexin V-binding (n=3). Insert: representative western blots of NDPK-D expression in HeLa cells. (b) LC-MS-based relative amounts of CL in OMM and IMM in HeLa cells expressing w/t NDPK-D and R90D mutant. Data are means±S.D., n=3. *P<0.05 compared with cells without CCCP treatment, #P<0.05 compared with cells expressing w/t NDPK-D under the same conditions (20 μM CCCP/4 h)