Figure 2.
DNA Methylation Changes in Fibroblasts after miR-29a Depletion
(A) The number of hyper- and hypo-DMRs in D551 fibroblasts depleted of miR-29a (antagomir-mediated, samples collected for MeDIP and hMeDIP 3 days post-transfection) and human ESCs.
(B) Venn diagrams show overlap of hyper- and hypo-DMRs of 5hmC between miR-29a-depleted cells and human ESCs.
(C) The distribution of hyper- and hypo-DMRs of 5hmC and 5mC around the gene body and CpGI in miR-29a-depleted cells and human ESCs.
(D) GO analysis of 5mC- and 5hmC-enriched regions specific to control and miR-29ai. Venn diagrams show the number of genes regulated by 5hmC and 5mC in miR-29a depleted and control cells. We did not detect any significant GO terms in genes regulated by 5hmC.
(E) NANOG undergoes demethylation and CDH2 undergoes methylation in miR-29a-depleted cells. These loci become similar to those in human ESCs.