Figure 3. Knockdown of LATS1 promotes cell growth and invasion.
(A) LATS1 mRNA and (B) protein expression were respectively examined by Real-time PCR and Western blotting in BGC-823 and AGS cells transfected with shLATS1. (C) The effect of LATS1 knockdown on cell proliferative activity was evaluated by MTT assay, showing that LATS1 knockdown prompted cell growth after shLATS1 transfection for 72 h. (D) Representative micrographs of Transwell invasion assay were indicated in BGC-823 and AGS cells transfected with shLATS1 (magnification, 200×). (E) Knockdown of LATS1 enhanced cell invasive potential compared with the NC group. (F) Flow cytometry analysis of early apoptotic rate in BGC-823 and AGS cells after shLAST1 treatment for 48 h. (G) Knockdown of LATS1 cut down cell apoptosis compared with the NC group. (H) Flow cytometry analysis of cell cycle distribution in BGC-823 and AGS cells treated with shLATS1. (I) Knockdown of LATS1 decreased the cell cycle arrest at S phase compared with the NC group. The data were shown as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01.