FIGURE 5.
Immunofluorescence of dystrophin distribution in human hippocampus of a post-mortem control patient (A,C,E) and a TLE patient without sclerosis (B,D,F). (A) NeuN (red) in the GCL of the DG without dystrophin (green) positive astroglial-like cells in post mortem control tissue. Next, none of these NeuN positive cells are stained yellow and are therefore dystrophin negative, 100 times magnification. (B) NeuN staining revealing NeuN (red) in the GCL of the DG and many dystrophin (green) positive astroglial-like cells in the hippocampus (DG) of a TLE patient, 100 times magnification. Again, no colocalization is observed. (C) GFAP (red) but not dystrophin (green) positive astrocytes (indicated by means of black arrowheads) around the DG in the hippocampus of a post-mortem control patient, 200 times magnification. (D) Dystrophin positive astrocytes around the DG of an epileptic hippocampus, revealing clear (yellow) colocalization, double stained for dystrophin (green) and GFAP (red), 200 times magnification. (E) 400 times magnified close-up of the DG of a post-mortem control patient. The white arrowhead indicates a glial cell that reveals some colocalization between GFAP (red) and dystrophin (green), whilst the black arrowheads indicate none colocalizing astrocytes. (F) 400 times magnified close-up of an area within the DG of a TLE patient. The white arrowheads depicted indicate, again, glial cells that show (yellow) colocalization between GFAP (red) and dystrophin (green). Asterisks indicate dystrophin positive blood vessels.