FIGURE 6.
Loss of Irg1 decreases itaconate and succinate levels in Irg1 KO BMDMs. a, Irg1 expression levels in LPS-activated (24 h, 10 ng·ml−1 LPS) BMDMs obtained from Irg1 KO and WT mice. Error bars represent expression levels obtained from two independent mice (mean ± S.E.) relative to L27. b and c, itaconate and succinate levels in BMDMs obtained from Irg1 KO and WT mice. Cells were activated for 6 h with 10 ng·ml−1 LPS. Error bars represent mean ± S.E. of metabolite levels [mm] of six biological replicates obtained from two independent mice.