Figure 2. Effect of claudin-2 KO on renal Na+ handling.
(A) Effect of dietary Na+ depletion. Urine Na excretion rate, expressed as the ratio of Na to creatinine (Cr) concentration (mean ± SEM, n = 4–5 per group), is shown in mice on a normal (0.3%) Na diet (day 1 to day 5), followed by a Na+-deficient (0.01%) diet (day 6 to day 9). (B) Furosemide (Fur) challenge test. Mice were administered vehicle (Veh) i.p. on the first day and furosemide 25 mg/kg on the second day. Urine Na+ excretion after furosemide was greater in KO mice than in WT mice. *P < 0.05, by paired Student’s t test (n = 7–9 per group).