Fig. 7.
TWEAK downregulates SIRT3 in cultured tubular cells and this is prevented by crotonate. (A) SIRT3 mRNA levels in tubular cells incubated with 100 ng/ml TWEAK. Mean±s.e.m. of three independent experiments. *P<0.05 vs control (non-parametric Mann–Whitney U-test). (B) Total protein levels of SIRT3 in MCT cells incubated with 100 ng/ml TWEAK for different periods of time. Mean±s.e.m. of three independent experiments. *P<0.05 vs control (non-parametric Mann–Whitney U-test). (C) Kidney SIRT3 mRNA levels in mice treated with TWEAK. Mean±s.e.m. of five animals per group. *P<0.05 vs control (non-parametric Mann–Whitney U-test). (D) Kidney SIRT3 protein levels in mice treated with TWEAK for different times. Mean±s.e.m. of five animals per group. *P<0.05 vs control (non-parametric Mann–Whitney U-test). (E) SIRT3 mRNA levels were tested in tubular cells incubated with 100 ng/ml TWEAK with or without 50 mM crotonate for 6 h. Data from three independent experiments and expressed as mean±s.e.m. *P<0.05 vs vehicle control; #P<0.05 vs vehicle TWEAK (non-parametric Mann–Whitney U-test). (F) Total SIRT3 protein levels in tubular cells incubated with 100 ng/ml TWEAK with or without 50 mM crotonate for 48 h. Data from four independent experiments and expressed as mean±s.e.m. *P<0.05 vs vehicle control, #P<0.05 vs vehicle TWEAK (non-parametric Mann–Whitney U-test).